A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Manimegalai, S.
- Safety of Uv-selected Helicoverpa armigera Nucleopolyhedrovirus to Non-target Beneficial Organisms
Authors
1 Department of Agricultural Entomology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 107-112Abstract
The safety of the UV-selected Coimbatore isolate of Helicoverpa armigera nucleopolyhedrovirus (HaNPV-UVT-CBEI) was tested against the non-target organisms viz., Trichogramma chilonis, Chrysoperla carnea, honey bee species and Bombyx mori to find out whether the cyclical exposure of HaNPV to ultra-violet (UV) radiation could cause adverse effect on the above organisms. The results showed that the HaNPV-UVT-CBEI had no adverse effect on the growth parameters, survival and production parameters of the organisms tested in comparison with the UV shielded HaNPV (HaNPV-UVS-CBEI). The cyclical exposure of HaNPV to UV radiation for the selection of UV tolerant strain did not have any deleterious effect on the safety of the virus to the non-target organisms.Keywords
Apis sp., Bombyx mori, Chrysoperla carnea, Helicoverpa armigera, Nucleopolyhedrovirus, Safety, Trichogramma chilonis.- Scope for Biological Control of Powdery Mildew of Mulberry with Illeis bielawskii Ghorpade, a Mycophagous Coccinellid
Authors
1 Department of Sericulture, Centre for Plant Protection Studies Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 327-331Abstract
Mulberry powdery mildew disease caused by Phyllactinia corylea (Pers.) Karst is a production constraint during winter months. A mycophagous coccinellid, Illeis bielawskii Ghorpade was found to be associated with the disease. Population of I. bielawskii among mulberry varieties ranged from 2.47numbers/plant (MR2) to 15.92/plant (Kanva 2). The egg, larval and pupal periods were 2.97, 9.00 and 3.22 days, respectively. The adult longevity was 20-25 days. The grubs and beetles actively fed on the fungus and the rate of feeding was found to be highest in adult (118.91 cm2), followed by fourth instar grub (43.28 cm2), Safety studies conducted revealed that there was no significant difference in hatching of eggs between control (no insecticide treatment) and the insecticide treatments, viz., malathion, methyl demeton and dichlorvos, However, a mortality of 50 and 46.67 per cent was observed when grubs were fed with mildew affected leaves treated with malathion and dichlorvos. To be effective as a biocontrol agent, it should not behave as a vector, Studies conducted on disease-vector relationship revealed that I.bielawskii does not have any significant role in disseminating the powdery mildew pathogen, P. corylea.Keywords
Feeding Rate, Grub Mortality, Hatchability, Illeis bielawskii, Phyllactinia corylea, Mulberry, Vector.- Effect of Combinations of Vairimorpha necatrix (Kramer) with Antibiotics on the Susceptibility of Spodoptera litura (Fabricius)
Authors
1 Department of Agricultural Entomology Tamil NaduAgricultural University Coimbatore 641 003, Tamil Nadu, IN
2 Department of Agricultural Entomology Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 20, No 1 (2006), Pagination: 97-100Abstract
Vairimorpha necatrix (Kramer) was combined with different antibiotics to increase its pathogenicity. It was administered to third instar larvae of Spodoptera litura (Fabricius) under laboratory conditions by diet surface treatment and the mortality and spore yield was studied. Reproductive spores of V.necatrix at 106 spores/ml were mixed with 100 ppm of antibiotics viz. gentamycin, oxytetracycline, ampicillin, kana mycin and chloramphenicol. The results showed that the larval mortality due to various Vairimorpha antibiotic combinations ranged from 10 to 62.07 per cent and it was high compared to prepupal and pupal stages. Assessment of spore yield in the infected larvae, pre-pupae and pupae revealed that larval stages recorded higher production of spores which ranged from 0.23 to 2.36 x 1010 spores for larva, 2.26 to 5.28 x 109 spores for pre pupa and 0.28 to 2.23 x 109 spores for pupa. Among the various combinations, Vairimorpha-gentamycin combination produced higher mortality and spore yield of V. necatrix.Keywords
Antibiotics, Mortality, Spodoptera litura, Spore Yield, Vairimorpha necatrix.- Metabolome heterogeneity in the isolates of entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin
Authors
1 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
2 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
3 Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 33, No 4 (2019), Pagination: 326-335Abstract
Entomopathogenic fungi are known to produce a multitude of low molecular weight secondary metabolites involved in different biological processes including fungal development, intercellular communication and interaction with other organisms in complex niches. In the present investigation, heterogeneity in metabolome profile of three isolates of Beauveria bassiana viz., MH590235 (TM), MK918495 (BR) and KX263275 (BbI8) were analyzed through GC-MS. Distinct differences in metabolite profile of the isolates were observed. A total of 63 metabolites were detected from all the isolates combined. Metabolites, 5-Oxotetrahydrofuran-2-carboxylic acid and undecane were found to be specific to BR isolate. Macrocyclic gamma lactones were detected in culture filtrates of BR and BbI8, oleic acid and hexadecanoic acid in TM and BR. An insecticidal compound, levoglucos an was detected in all the fungal isolates. Among the isolates, TM revealed higher variability in the metabolite production through PCA analysis. The metabolome of TM isolate contained compounds having several biological functions, viz., insecticidal and antimicrobial activity, lipid and fatty acid metabolisms and virulence enhancing factors.
Keywords
Beauveria bassiana, Biological Functions, GC-MS, Metabolome Heterogeneity, PCA Analysis.References
- Amiri-Besheli B, Khambay B, Cameron S, Deadman ML, Butt T.M. 2000. Inter- and intra-specific variation in destruxin production by insect pathogenic Metarhizium spp., and its significance to pathogenesis. Mycol Res. 104: 447-452. https://doi.org/10.1017/S095375629900146X https://doi.org/10.1017/S095375629900146X
- Bernabé M, Salvachúa D, Jiménez-Barbero J, Leal JA, Prieto A. 2011. Structures of wall heterogalactomannans isolated from three genera of entomopathogenic fungi. Fungal Biol. 115(9): 862-870. https://doi.org/10.1016/j.funbio.2011.06.015 https://doi.org/10.1016/j.funbio.2011.06.015 PMid:21872183
- Brakhage AA. 2013 Regulation of fungal secondary metabolism. Nat Rev Microbiol. 11(1):21-32. https://doi.org/10.1038/nrmicro2916 https://doi.org/10.1038/nrmicro2916 PMid:23178386
- Brennan PJ, Griffin PF, Lösel DM, Tyrrell D. 1975. The lipids of fungi. Prog Chem Fats Lipids 14: 49-89. https://doi.org/10.1016/0079-6832(75)90002-6
- Butt TM, Jackson CW, Magan N. 2001. Fungi as Biocontrol Agents: Potential, Progress and Problems. CAB International, Wallingford. https://doi.org/10.1079/9780851993560.0000
- deBekker C, Smith PB, Patterson AD, Hughes DP. 2013. Metabolomics reveals the heterogeneous secretome of two entomopathogenic fungi to ex vivo cultured insect tissues. PLoS ONE 8(8): e70609. https://doi.org/10.1371/journal.pone.0070609 PMid:23940603 PMCid:PMC3734240
- Domon K, Keiji T, Yutaka O, Junichiro B, Kei K, Akira W, Masaaki K, Takeshi M, Seisuke I. 2018. “Alkyl phenyl sulfide derivative and pest control agent.” U.S. Patent Application 10/023,532, filed July 17, 2018.
- Douglas CM. 2001. Fungal β (1, 3)-D-glucan synthesis. Sabouraudia 39(1): 55-66. https://doi.org/10.1080/ mmy.39.1.55.66 https://doi.org/10.1080/mmy.39.1.55.66 PMid:11800269
- Gowri PM, Haribabu K, Kishore H, Manjusha O, Biswas S, Murty USN. 2011. Microbial transformation of(+)heraclenin by Aspergillus niger and evaluation of its antiplasmodial and antimicrobial activities. Current Sci. 100(11):1706-1711.
- Hölldobler B, Wilson EO. 1990. The Ants. Harvard University Press, US. https://doi.org/10.1007/978-3-662-10306-7 PMid:24263721
- Hyun SH, Lee SY, Sung GH, Kim SH, Choi HK. 2013. Metabolic Profiles and Free Radical Scavenging Activity of Cordyceps bassiana Fruiting Bodies According to Developmental Stage. PLoS ONE. 8(9): e73065. https://doi.org/10.1371/journal.pone.0073065 PMid:24058459 PMCid:PMC3772819
- Retrieved from: https://pubchem.ncbi.nlm.nih.gov/ compound/Digitoxin
- Kadowaki M, Godoy M, Kumagai P, Costa-Filho A, Mort A, Prade R, Polikarpov I. 2018. Characterization of a new glyoxal oxidase from the thermophilic fungus Myceliophthora thermophila M77: hydrogen peroxide production retained in 5-hydroxymethylfurfural oxidation. Catalysts 8(10): 476. https://doi.org/10.3390/catal8100476.
- Keller NP. 2015. Translating biosynthetic gene clusters into fungal armor and weaponry. Nat Chem Biol.
- (9):671-677. https://doi.org/10.1038/nchembio.1897 PMid:26284674 PMCid:PMC4682562
- Kershaw MJ, Moorhouse ER, Bateman RP, Reynolds SE, Charnley AK. 1999. The role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insect. J Invertebr Pathol. 74: 213-223. https://doi.org/10.1006/jipa.1999.4884 PMid:10534408
- Liu H, Zhao X, Guo M, Liu H, Zheng Z. 2015. Growth and metabolism of Beauveria bassiana spores and mycelia. BMC Microbiology 15(1): 267. https://doi.org/10.1186/s12866-015-0592-4 PMid:26581712 PMCid:PMC4652391
- Liu L, Liu S, Chen X, Guo, L, Che Y. 2009. Pestalofones A-E, bioactive cyclohexanone derivatives from the plant endophytic fungus Pestalotiopsis fici. Bioorg Med Chem. 17: 606-613. https://doi.org/10.1016/j.bmc.2008.11.066 PMid:19101157
- Mazet I, Vey A. 1995. Hirsutellin A, a toxic protein produced in vitro by Hirsutella thompsonii. Microbiology 141(6): 1343-1348. https://doi.org/10.1099/13500872-141-6-1343 PMid:7670635
- Mil-Homens D, Bernardes N, Fialho AM. 2012. The antibacterial properties of docosahexaenoic omega-3 fatty acid against the cystic fibrosis multiresistant pathogen Burkholderia cenocepacia. FEMS Microbiol Lett. 328(1): 61-69. https://doi.org/10.1111/j.1574-6968.2011.02476.x PMid:22150831
- Oh TJ, Hyun SH, Lee SG, Chun YJ, Sung GH. 2014. NMR and GC-MS based metabolic profiling and free-radical scavenging activities of Cordyceps pruinosa mycelia cultivated under different media and light conditions. PLoS ONE 9(3): e90823. https://doi.org/10.1371/journal.pone.0090823 PMid:24608751 PMCid:PMC3946585
- Oller-López JL, Iranzo M, Mormeneo S, Oliver E, Cuerva JM, Oltra JE. 2005. Bassianolone: an antimicrobial precursor of cephalosporolides E and F from the entomoparasitic fungus Beauveria bassiana. Org Biomol Chem. 3(7): 1172-1173. https://doi.org/10.1039/B417534D PMid:15785802
- Ortiz-Urquiza A, Fan Y, Garrett T, Keyhani NO. 2016. Growth substrates and caleosin-mediated functions affect conidial virulence in the insect pathogenic fungus Beauveria bassiana. Microbiology 162(11): 1913-1921. https://doi.org/10.1099/mic.0.000375 https://doi.org/10.1099/mic.0.000375 PMid:27655425
- Paulraj MG, Reegan AD, Ignacimuthu S. 2011. Toxicity of Benzaldehyde and Propionic Acid against Immatureand Adult Stages of Aedes aegypti (Linn.) and Culex quinquefasciatus (Say) (Diptera: Culicidae). J Entomol. 8: 539-547. https://doi.org/10.3923/je.2011.539.547
- Ragavendran C, Dubey NK, Natarajan D. 2017. Beauveria bassiana (Clavicipitaceae): a potent fungal agent for controlling mosquito vectors of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). RSC Advances. 7(7): 3838-3851. https://doi.org/10.1039/C6RA25859J
- Ramadan Z, Jacobs D, Grigorov M, Kochhar S. 2006. Metabolic profiling using principal component analysis, discriminant partial least squares, and genetic algorithms. Talanta 68: 1683-1691. https://doi.org/10.1016/j.talanta.2005.08.042 PMid:18970515
- Rohrlich C, Merle I, MzeHassani I, Verger M, Zuin M, Besse S. 2018. Variation in physiological host range in three strains of two species of the entomopathogenic fungus Beauveria. PLoS ONE 13(7): e0199199. https://doi.org/10.1371/journal.pone.0199199 PMid:29975710 PMCid:PMC6033404
- Sayed AM, Behle RW, Tiilikkala K, Vaughn SF. 2018. Insecticidal activity of bio-oils and biochar as pyrolysis products and their combination with microbial agents against Agrotis ipsilon (Lepidoptera: Noctuidae). Pestic Phytomed. 33(1): 39-52. https://doi.org/10.2298/PIF1801039S
- Smedsgaard J. 1997. Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures. J Chromatogr A 760(2): 264-270. https://doi.org/10.1016/s0021-9673(96)00803-5
- Strasser H, Abendstein D, Stuppner H, Butt TM. 2000. Monitoring the distribution of secondary metabolites produced by the entomogenous fungus Beauveria brongniartii with particular reference to oosporein. Mycol Res. 104: 1227-1233. https://doi.org/10.1017/S0953756200002963
- Strasser H, Vey A, Butt TM. 2000. Are there any risks in using entomopathogenic fungi for pest control, with particular reference to the bioactive metabolites of Metarhizium, Tolypocladium and Beauveria species? Biocontrol Sci Technol. 10: 717-735. https://doi.org/10.1080/09583150020011690
- Talaei-Hassanloui R, Kharazi-Pakdel A, Goettel M, Mozaffari J. 2006. Variation in virulence of Beauveria bassiana isolates and its relatedness to some morphological characteristics. Biocontrol Sci Technol. 16(5): 525534. https://doi.org/10.1080/09583150500532758
- Valero-Jiménez CA, Debets AJ, van Kan JA, Schoustra SE, Takken W, Zwaan BJ. 2014. Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes. Malar J. 13(1):1-8. https://doi.org/10.1186/1475-2875-13-479 PMid:25480526 PMCid:PMC4364330
- Vey A, Hoagland R, Butt TM. 2001. Toxic metabolites of fungal biocontrol agents, pp. 311-345. In: Butt TM, Jackson CW. and Magan N. (Eds.). Fungi as Biocontrol Agents: Potential, Progress and Problems. CAB International, Wallingford, UK. https://doi.org/10.1079/9780851993560.0311
- Vivekanandhan P, Kavitha T, Karthi S, Senthil-Nathan S, Shivakumar M. 2018. Toxicity of Beauveria bassiana-28 mycelial extracts on larvae of Culex quinquefasciatus mosquito (Diptera: Culicidae). Int J Environ Res Public Health 15(3): 440. https://doi.org/10.3390/ijerph15030440 PMid:29510502 PMCid:PMC5876985
- Wakil W, Yasin M, Shapiro-Ilan D. 2017. Effects of single and combined applications of entomopathogenic fungi and nematodes against Rhynchophorus ferrugineus (Olivier). Sci Rep. 7(1): 5971. https://doi.org/10.1038/s41598-017-05615-3 PMid:28729649 PMCid:PMC5519636
- Wahlman M, Davidson BS. 1993. New destruxins from the entomopathogenic fungus Metarhizium anisopliae. J Nat Prod. 56(4): 643-647. https://doi.org/10.1021/np9601216
- Wang CS, Skrobek A, Butt TM. 2004. Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae in liquid and solid media. J Invertebr Pathol. 85: 168-174. https://doi.org/10.1016/j.jip.2004.02.008 PMid:15109899
- Woappi Y, Gabani P, Singh A, Singh O.V. 2016. Antibiotrophs: the complexity of antibiotic-subsisting and antibioticresistant microorganisms. Crit Rev Microbial. 42(1): 17-30. https://doi.org/10.3109/1040841X.2013.875982 PMid:24495094
- Xu Y, Orozco R, Wijeratne EK, Espinosa-Artiles P, Gunatilaka AL, Stock SP, Molnár I. 2009. Biosynthesis of the cyclooligomer depsipeptide bassianolide, an insecticidal virulence factor of Beauveria bassiana. Fungal Genet Biol. 46(5): 353-364. https://doi.org/10.1016/j.fgb.2009.03.001 PMid:19285149
- Xu Y, Orozco R, Wijeratne EMK, Gunatilaka AAL, Stock SP, Molnár I. 2008. Biosynthesis of the cyclooligomer depsipeptide beauvericin, a virulence factor of the entomopathogenic fungus Beauveria bassiana. Chem Biol. 15: 898-907. https://doi.org/10.1016/j.chembiol.2008.07.011 PMid:18804027
- Zhang S, Widemann E, Bernard G, Lesot A, Pinot F, Pedrini N, Keyhani NO. 2012. CYP52X1, representing new cytochrome P450 subfamily, displays fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus Beauveria bassiana. J Biol Chem. 287(16): 13477-13486. https://doi.org/10.1074/jbc.M111.338947 aPMid:22393051 PMCid:PMC3339963